Part:BBa_K3132019

8*Flag

Flag-tag is a segment composed of 8 amino acid residues, playing a role of labeling in protein expression and location. As a fusion expression label, flag usually does not interact with the target protein and usually does not affect the functional properties of the target protein. As it can be recognized by anti-flag antibodies, we hope fused several flag tags to IL-15 protein will enhance the ability of inducing receptor dimerization. Here we fused 8 flags to IL-15. Moreover, to determine the best amount of these peptide tags, we set different enzyme cutting sites between every 2 flag single chains, after cutting with different restriction enzymes, we got the other three types of IL-15 proteins labeled with different lengths of flags: IL-15-flag6×, IL-15-flag4×, IL-15-flag2×.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 280
  Illegal NheI site found at 583
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 595
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 73
  Illegal SapI.rc site found at 223
  Illegal SapI.rc site found at 373